| 质粒名称: | pCI neo-hEST2 |
|---|---|
| 出品公司： | addgene |
| 目录编号: | 1781 |
| 存储实验室: | Bob Weinberg |
|
载体骨架基本信息 |
|
| 载体名称: | pCI neo |
| 空载体大小: | 5400 bp |
| 完整质粒大小: | 8898bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | hTERT/Ever Shorter Telomeres 2/elomerase reverse transcriptase/pCI-hTERT |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 3450bp |
| 基因突变: | T2A, S13L, A92T, V104T, V114I, E161K, Y193K, F198Y, D202T, I203V, Y258F, A283V, K288T, N290Y relative to RpBphP2 |
| GenBank编号 | AF018167 |
| 启动子: | CMV |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | EcoRI(未破坏) |
| 3’克隆位点: | SalI (未破坏) |
| 5’测序引物 | T7 |
| 3’测序引物 | EBV-rev |
|
文献引用方法 |
|
| 材料和方法部分: | pCI neo-hEST2 was a gift from Bob Weinberg (Addgene plasmid # 1781 ; http://n2t.net/addgene:1781 ; RRID:Addgene_1781) |
| 参考文献部分: | hEST2, the putative human telomerase catalytic subunit gene, is up-regulated in tumor cells and during immortalization. Meyerson M, Counter CM, Eaton EN, Ellisen LW, Steiner P, Caddle SD, Ziaugra L, Beijersbergen RL, Davidoff MJ, Liu Q, Bacchetti S, Haber DA, Weinberg RA. Cell 1997 Aug 22;90(4):785-95. 10.1016/S0092-8674(00)80538-3 PubMed 9288757 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCI neo-hEST2 8898 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pCI neo-hEST2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8898)
AUTHORS Meyerson M, Counter CM, Eaton EN, Ellisen LW, Steiner P, Caddle SD,
Ziaugra L, Beijersbergen RL, Davidoff MJ, Liu Q, Bacchetti S, Haber
DA, Weinberg RA
TITLE hEST2, the putative human telomerase catalytic subunit gene, is
up-regulated in tumor cells and during immortalization.
JOURNAL Cell 1997 Aug 22;90(4):785-95.
PUBMED 9288757
REFERENCE 2 (bases 1 to 8898)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8898
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 108..696
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 597..616
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
enhancer 907..1286
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 1287..1498
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 1449..1469
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 1495..1519
/label=LNCX
/note="Human CMV promoter, forward primer"
intron 1659..1791
/label=chimeric intron
/note="chimera between introns from human beta-globin and
immunoglobulin heavy chain genes"
primer_bind 1774..1793
/label=pMT2-F
/note="Synthetic intron, forward primer"
promoter 1836..1854
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 1916..5314
/codon_start=1
/gene="TERT"
/product="human telomerase reverse transcriptase, the
catalytic subunit of telomerase"
/label=hTERT
/note="prevents telomere shortening and allows
postsenescent cells to proliferate beyond crisis (Counter
et al., 1998); also known as EST2"
/translation="MPRAPRCRAVRSLLRSHYREVLPLATFVRRLGPQGWRLVQRGDPA
AFRALVAQCLVCVPWDARPPPAAPSFRQVSCLKELVARVLQRLCERGAKNVLAFGFALL
DGARGGPPEAFTTSVRSYLPNTVTDALRGSGAWGLLLRRVGDDVLVHLLARCALFVLVA
PSCAYQVCGPPLYQLGAATQARPPPHASGPRRRLGCERAWNHSVREAGVPLGLPAPGAR
RRGGSASRSLPLPKRPRRGAAPEPERTPVGQGSWAHPGRTRGPSDRGFCVVSPARPAEE
ATSLEGALSGTRHSHPSVGRQHHAGPPSTSRPPRPWDTPCPPVYAETKHFLYSSGDKEQ
LRPSFLLSSLRPSLTGARRLVETIFLGSRPWMPGTPRRLPRLPQRYWQMRPLFLELLGN
HAQCPYGVLLKTHCPLRAAVTPAAGVCAREKPQGSVAAPEEEDTDPRRLVQLLRQHSSP
WQVYGFVRACLRRLVPPGLWGSRHNERRFLRNTKKFISLGKHAKLSLQELTWKMSVRGC
AWLRRSPGVGCVPAAEHRLREEILAKFLHWLMSVYVVELLRSFFYVTETTFQKNRLFFY
RKSVWSKLQSIGIRQHLKRVQLRELSEAEVRQHREARPALLTSRLRFIPKPDGLRPIVN
MDYVVGARTFRREKRAERLTSRVKALFSVLNYERARRPGLLGASVLGLDDIHRAWRTFV
LRVRAQDPPPELYFVKVDVTGAYDTIPQDRLTEVIASIIKPQNTYCVRRYAVVQKAAHG
HVRKAFKSHVSTLTDLQPYMRQFVAHLQETSPLRDAVVIEQSSSLNEASSGLFDVFLRF
MCHHAVRIRGKSYVQCQGIPQGSILSTLLCSLCYGDMENKLFAGIRRDGLLLRLVDDFL
LVTPHLTHAKTFLRTLVRGVPEYGCVVNLRKTVVNFPVEDEALGGTAFVQMPAHGLFPW
CGLLLDTRTLEVQSDYSSYARTSIRASLTFNRGFKAGRNMRRKLFGVLRLKCHSLFLDL
QVNSLQTVCTNIYKILLLQAYRFHACVLQLPFHQQVWKNPTFFLRVISDTASLCYSILK
AKNAGMSLGAKGAAGPLPSEAVQWLCHQAFLLKLTRHRVTYVPLLGSLRTAQTQLSRKL
PGTTLTALEAAANPALPSDFKTILD"
promoter complement(5336..5353)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
polyA_signal 5371..5492
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5382..5401)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind 5436..5455
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
rep_origin 5678..6133
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5765..5784)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5975..5996
/label=F1ori-F
/note="F1 origin, forward primer"
promoter 6250..6607
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(6273..6293)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 6458..6593
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 6520..6539
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 6658..7452
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(6712..6731)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 7322..7341
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 7516..7564
/note="synthetic polyadenylation signal"
primer_bind complement(7605..7624)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 7724..7746
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(7784..7802)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 7870..7974
/gene="bla"
/label=AmpR promoter
CDS 7975..8835
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(8193..8212)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
ORIGIN
1 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
61 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
121 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
181 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
241 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
301 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
361 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
421 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
481 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
541 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
601 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
661 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
721 cggttcctgg ccttttgctg gccttttgct cacatggctc gacagatctt caatattggc
781 cattagccat attattcatt ggttatatag cataaatcaa tattggctat tggccattgc
841 atacgttgta tctatatcat aatatgtaca tttatattgg ctcatgtcca atatgaccgc
901 catgttggca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
961 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
1021 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
1081 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
1141 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
1201 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
1261 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
1321 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
1381 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactgc gatcgcccgc
1441 cccgttgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg
1501 tttagtgaac cgtcagatca ctagaagctt tattgcggta gtttatcaca gttaaattgc
1561 taacgcagtc agtgcttctg acacaacagt ctcgaactta agctgcagtg actctcttaa
1621 ggtagccttg cagaagttgg tcgtgaggca ctgggcaggt aagtatcaag gttacaagac
1681 aggtttaagg agaccaatag aaactgggct tgtcgagaca gagaagactc ttgcgtttct
1741 gataggcacc tattggtctt actgacatcc actttgcctt tctctccaca ggtgtccact
1801 cccagttcaa ttacagctct taaggctaga gtacttaata cgactcacta taggctagcc
1861 tcgagaattc gtcctgctgc gcacgtggga agccctggcc ccggccaccc ccgcgatgcc
1921 gcgcgctccc cgctgccgag ccgtgcgctc cctgctgcgc agccactacc gcgaggtgct
1981 gccgctggcc acgttcgtgc ggcgcctggg gccccagggc tggcggctgg tgcagcgcgg
2041 ggacccggcg gctttccgcg cgctggtggc ccagtgcctg gtgtgcgtgc cctgggacgc
2101 acggccgccc cccgccgccc cctccttccg ccaggtgtcc tgcctgaagg agctggtggc
2161 ccgagtgctg cagaggctgt gcgagcgcgg cgcgaagaac gtgctggcct tcggcttcgc
2221 gctgctggac ggggcccgcg ggggcccccc cgaggccttc accaccagcg tgcgcagcta
2281 cctgcccaac acggtgaccg acgcactgcg ggggagcggg gcgtgggggc tgctgctgcg
2341 ccgcgtgggc gacgacgtgc tggttcacct gctggcacgc tgcgcgctct ttgtgctggt
2401 ggctcccagc tgcgcctacc aggtgtgcgg gccgccgctg taccagctcg gcgctgccac
2461 tcaggcccgg cccccgccac acgctagtgg accccgaagg cgtctgggat gcgaacgggc
2521 ctggaaccat agcgtcaggg aggccggggt ccccctgggc ctgccagccc cgggtgcgag
2581 gaggcgcggg ggcagtgcca gccgaagtct gccgttgccc aagaggccca ggcgtggcgc
2641 tgcccctgag ccggagcgga cgcccgttgg gcaggggtcc tgggcccacc cgggcaggac
2701 gcgtggaccg agtgaccgtg gtttctgtgt ggtgtcacct gccagacccg ccgaagaagc
2761 cacctctttg gagggtgcgc tctctggcac gcgccactcc cacccatccg tgggccgcca
2821 gcaccacgcg ggccccccat ccacatcgcg gccaccacgt ccctgggaca cgccttgtcc
2881 cccggtgtac gccgagacca agcacttcct ctactcctca ggcgacaagg agcagctgcg
2941 gccctccttc ctactcagct ctctgaggcc cagcctgact ggcgctcgga ggctcgtgga
3001 gaccatcttt ctgggttcca ggccctggat gccagggact ccccgcaggt tgccccgcct
3061 gccccagcgc tactggcaaa tgcggcccct gtttctggag ctgcttggga accacgcgca
3121 gtgcccctac ggggtgctcc tcaagacgca ctgcccgctg cgagctgcgg tcaccccagc
3181 agccggtgtc tgtgcccggg agaagcccca gggctctgtg gcggcccccg aggaggagga
3241 cacagacccc cgtcgcctgg tgcagctgct ccgccagcac agcagcccct ggcaggtgta
3301 cggcttcgtg cgggcctgcc tgcgccggct ggtgccccca ggcctctggg gctccaggca
3361 caacgaacgc cgcttcctca ggaacaccaa gaagttcatc tccctgggga agcatgccaa
3421 gctctcgctg caggagctga cgtggaagat gagcgtgcgg ggctgcgctt ggctgcgcag
3481 gagcccaggg gttggctgtg ttccggccgc agagcaccgt ctgcgtgagg agatcctggc
3541 caagttcctg cactggctga tgagtgtgta cgtcgtcgag ctgctcaggt ctttctttta
3601 tgtcacggag accacgtttc aaaagaacag gctctttttc taccggaaga gtgtctggag
3661 caagttgcaa agcattggaa tcagacagca cttgaagagg gtgcagctgc gggagctgtc
3721 ggaagcagag gtcaggcagc atcgggaagc caggcccgcc ctgctgacgt ccagactccg
3781 cttcatcccc aagcctgacg ggctgcggcc gattgtgaac atggactacg tcgtgggagc
3841 cagaacgttc cgcagagaaa agagggccga gcgtctcacc tcgagggtga aggcactgtt
3901 cagcgtgctc aactacgagc gggcgcggcg ccccggcctc ctgggcgcct ctgtgctggg
3961 cctggacgat atccacaggg cctggcgcac cttcgtgctg cgtgtgcggg cccaggaccc
4021 gccgcctgag ctgtactttg tcaaggtgga tgtgacgggc gcgtacgaca ccatccccca
4081 ggacaggctc acggaggtca tcgccagcat catcaaaccc cagaacacgt actgcgtgcg
4141 tcggtatgcc gtggtccaga aggccgccca tgggcacgtc cgcaaggcct tcaagagcca
4201 cgtctctacc ttgacagacc tccagccgta catgcgacag ttcgtggctc acctgcagga
4261 gaccagcccg ctgagggatg ccgtcgtcat cgagcagagc tcctccctga atgaggccag
4321 cagtggcctc ttcgacgtct tcctacgctt catgtgccac cacgccgtgc gcatcagggg
4381 caagtcctac gtccagtgcc aggggatccc gcagggctcc atcctctcca cgctgctctg
4441 cagcctgtgc tacggcgaca tggagaacaa gctgtttgcg gggattcggc gggacgggct
4501 gctcctgcgt ttggtggatg atttcttgtt ggtgacacct cacctcaccc acgcgaaaac
4561 cttcctcagg accctggtcc gaggtgtccc tgagtatggc tgcgtggtga acttgcggaa
4621 gacagtggtg aacttccctg tagaagacga ggccctgggt ggcacggctt ttgttcagat
4681 gccggcccac ggcctattcc cctggtgcgg cctgctgctg gatacccgga ccctggaggt
4741 gcagagcgac tactccagct atgcccggac ctccatcaga gccagtctca ccttcaaccg
4801 cggcttcaag gctgggagga acatgcgtcg caaactcttt ggggtcttgc ggctgaagtg
4861 tcacagcctg tttctggatt tgcaggtgaa cagcctccag acggtgtgca ccaacatcta
4921 caagatcctc ctgctgcagg cgtacaggtt tcacgcatgt gtgctgcagc tcccatttca
4981 tcagcaagtt tggaagaacc ccacattttt cctgcgcgtc atctctgaca cggcctccct
5041 ctgctactcc atcctgaaag ccaagaacgc agggatgtcg ctgggggcca agggcgccgc
5101 cggccctctg ccctccgagg ccgtgcagtg gctgtgccac caagcattcc tgctcaagct
5161 gactcgacac cgtgtcacct acgtgccact cctggggtca ctcaggacag cccagacgca
5221 gctgagtcgg aagctcccgg ggacgacgct gactgccctg gaggccgcag ccaacccggc
5281 actgccctca gacttcaaga ccatcctgga ctgagtcgac ccgggcggcc gcttcccttt
5341 agtgagggtt aatgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca
5401 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
5461 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
5521 ttcaggttca gggggagatg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
5581 gtaaaatccg ataaggatcg atccgggctg gcgtaatagc gaagaggccc gcaccgatcg
5641 cccttcccaa cagttgcgca gcctgaatgg cgaatggacg cgccctgtag cggcgcatta
5701 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
5761 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
5821 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
5881 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
5941 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
6001 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
6061 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
6121 acgcttacaa tttcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
6181 gcatacgcgg atctgcgcag caccatggcc tgaaataacc tctgaaagag gaacttggtt
6241 aggtaccttc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa
6301 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
6361 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
6421 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
6481 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
6541 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
6601 ttgcaaaaag cttgattctt ctgacacaac agtctcgaac ttaaggctag agccaccatg
6661 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
6721 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
6781 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag
6841 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
6901 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
6961 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
7021 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
7081 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
7141 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc
7201 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
7261 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
7321 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
7381 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
7441 gagttcttct gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc
7501 catcacgatg gccgcaataa aatatcttta ttttcattac atctgtgtgt tggttttttg
7561 tgtgaatcga tagcgataag gatccgcgta tggtgcactc tcagtacaat ctgctctgat
7621 gccgcatagt taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
7681 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
7741 cagaggtttt caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta
7801 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg
7861 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
7921 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
7981 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
8041 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
8101 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
8161 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
8221 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
8281 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
8341 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
8401 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
8461 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
8521 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
8581 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
8641 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
8701 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
8761 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
8821 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
8881 cttcattttt aatttaaa
//
